1 Cooperation of p 40 phox with p 47 phox for Nox 2 activation during Fc γ R - mediated phagocytosis - - - Mechanism for acquisition of p 40 phox PI ( 3 ) P binding - - -
نویسندگان
چکیده
Takehiko Ueyama***, Junya Nakakita*, Takashi Nakamura*, Takeshi Kobayashi‡, Toshihiro Kobayashi†, Jeonghyun Son*, Megumi Sakuma*, Hirofumi Sakaguchi§, Thomas L. Leto¶, Naoaki Saito*** *Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe 657-8501, Japan; §Department of Otolaryngology-Head and Neck Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan; †Department of Physical Therapy, Kobe International University, Kobe; ‡Department of Physiology, Nagoya University Graduate School of Medicine, Nagoya; ¶Molecular Defenses Section, Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA
منابع مشابه
The phosphoinositide-binding protein p40phox activates the NADPH oxidase during FcγIIA receptor–induced phagocytosis
Superoxide produced by the phagocyte reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is essential for host defense. Enzyme activation requires translocation of p67(phox), p47(phox), and Rac-GTP to flavocytochrome b558 in phagocyte membranes. To examine the regulation of phagocytosis-induced superoxide production, flavocytochrome b558, p47(phox), p67(phox), and the FcgammaIIA...
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The assembly of cytosolic p47(phox) and p67(phox) with flavocytochrome b(558) at the membrane is crucial for activating the leukocyte NADPH oxidase that generates superoxide for microbial killing. p47(phox) and p67(phox) are linked via a high-affinity, tail-to-tail interaction involving a proline-rich region (PRR) and a C-terminal SH3 domain (SH3b), respectively, in their C-termini. This intera...
متن کاملAkt phosphorylates p47phox and mediates respiratory burst activity in human neutrophils.
Respiratory burst activity and phosphorylation of an NADPH oxidase component, p47(phox), during neutrophil stimulation are mediated by phosphatidylinositol 3-kinase (PI-3K) activation. Products of PI-3K activate several kinases, including the serine/threonine kinase Akt. The present study examined the ability of Akt to regulate neutrophil respiratory burst activity and to interact with and phos...
متن کاملFc gamma R-stimulated activation of the NADPH oxidase: phosphoinositide-binding protein p40phox regulates NADPH oxidase activity after enzyme assembly on the phagosome.
The phagocyte NADPH oxidase generates superoxide for microbial killing, and includes a membrane-bound flavocytochrome b(558) and cytosolic p67(phox), p47(phox), and p40(phox) subunits that undergo membrane translocation upon cellular activation. The function of p40(phox), which binds p67(phox) in resting cells, is incompletely understood. Recent studies showed that phagocytosis-induced superoxi...
متن کاملSH3-dependent assembly of the phagocyte NADPH oxidase
F ~hagocytic cells contain a complicated enzyme system, termed NADPH oxidase, that is responsible for the production of toxic oxygen species (1). The enzyme transfers electrons from NADPH to 02, forming O2-, which then can dismutate into H202. Subsequently, other oxygen derivatives, such as hydroxyl radical and hypochlorous acid, may be formed. Collectively, these oxygen products are toxic to c...
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